Journal: Journal of Translational Medicine

Article Title: C/D box small nucleolar RNA SNORD104 promotes endometrial cancer by regulating the 2ʹ-O-methylation of PARP1

doi: 10.1186/s12967-022-03802-z

Figure Lengend Snippet: SNORD104 upregulated PARP1 expression in vitro and in vivo. A Immunoprecipitation of SNORD104 using anti-FBL antibodies. B Immunoblot showing FBL protein levels in SNORD104-overexpressing HEC1B cells. C The 2ʹ-O-methylation level up-regulated RNA in the Nm-seq data set was intersected with the RNA data set of SNORD104-Blast, and the KEGG pathway enrichment analysis showed that the apoptotic pathway ranked first. D Anti-FBL antibody was used to perform RIP assay in HEC1B cells, and the mRNA enrichment of PARP1, ITPR3, TUBA1C, PARP4, LMNB1 was detected by qRT-PCR. E Evaluation of PARP1, ITPR3, TUBA1C, PARP4, LMNB1 protein levels after overexpression of SNORD104, and only PARP1 RNA and protein level were changed. F PARP1 mRNA and protein levels in SNORD104-knockdown Ishikawa cells. G SNORD104 and PARP1 mRNA expression in EC tissues (n = 71) and the correlation between the expression levels (R = 0.3222, P < 0.0001). H PARP1 protein levels in the nuclear fraction of SNORD104-overexpressing cells. I , J PARP1 protein levels in the SNORD104-overexpressing subcutaneous xenografts in nude mice. *P < 0.05, ns means P > 0.05

Article Snippet: Following overnight incubation with rabbit anti-FBL (1:2000 Magna, Proteintech, Rosemont, IL, USA), rabbit anti-PARP1 (1:2000 Magneto, Proteintech), rabbit anti-PARP4 (1:2000, Bioss, Woburn, MA, USA), anti-ITPR3 (1:2000, LSBio), anti-LMNB1 (1:10,000, Proteintech), anti-Vinculin (1:10,000, Proteintech), anti-GAPDH (1:5000, Proteintech) and anti-α-tubulin (TUBA1B/1C) (1:2000, Proteintech) antibodies at 4 ℃, the membranes were washed thrice with 1% Tris-buffered saline-Tween20 (TBST) buffer for 10 min each time.

Techniques: Expressing, In Vitro, In Vivo, Immunoprecipitation, Western Blot, Methylation, Quantitative RT-PCR, Over Expression

Journal: bioRxiv

Article Title: Glycan Profiling Identifies Chondroitin-4-sulfate as a Biomarker for Platinum Response and Therapeutic Target in Ovarian Cancer

doi: 10.1101/2025.10.06.675352

Figure Lengend Snippet: ( A ) Carboplatin and Triplatin chemical structures. ( B and C ) Cytotoxicity of carboplatin and Triplatin on human OVTOKO and JHOC5 cancer cell lines; 1h treatment. ( D ) Flow cytometry of 200 nM and 50 nM rVAR2-V5 binding to ES2 wt cells, ES2 wt cells + chABC treatment, or Xylt1/Xylt2 KO cells. ( E and F ) Cytotoxicity of carboplatin and Triplatin in ES2 wt and Xylt1/Xylt2 KO cell lines; 1h treatment. ( G and H ). Platinum cellular accumulation in ES2 wt and Xylt1/Xylt2 KO cells treated with 10 µM carboplatin or Triplatin for 1, 2, 4, and 8h. Platinum content was measured by inductively coupled plasma mass spectroscopy (ICP-MS) and normalized by number of cells. ( I and J ). Platinum-DNA adducts in ES2 wt and Xylt1/Xylt2 KO cells treated with 10 µM carboplatin or Triplatin 4, and 8h. ( K and M ) ES2-luc wt and Xylt1/Xylt2 KO tumors were implanted on the left and right flanks mice. Tumors were harvested after 24h treatment with 40 mg/kg i.p Carboplatin or 0.3 mg/kg i.p. Triplatin. Tumors were digested in nitric acid and platinum measured by ICP-MS.

Article Snippet: Bound peptide was detected with mouse anti-V5 antibody (R960-25, ThermoFisher; 1:700 in casein buffer, 45 min, room temperature), followed by Leica post-primary rabbit anti-mouse antibody (8 min) and goat anti-rabbit HRP conjugate (8 min), with PBS washes between each step.

Techniques: Flow Cytometry, Binding Assay, Clinical Proteomics, Mass Spectrometry

Journal: bioRxiv

Article Title: Glycan Profiling Identifies Chondroitin-4-sulfate as a Biomarker for Platinum Response and Therapeutic Target in Ovarian Cancer

doi: 10.1101/2025.10.06.675352

Figure Lengend Snippet: Representative images of rVAR2-V5 and H&E OC PDX staining and Qupath analysis. (C) Qupath segmentation of rVAR2-V5 (+), rVAR2-V5 (-), and necrotic tumor area in OC PDX models. (D) Sensitivity of OC PDX models to Triplatin and carboplatin. OC PDX models were treated i.p. with carboplatin (40 mg/kg) or Triplatin (0.3 mg/kg) on days 0, 4 and 8 (orange arrows). * * p<0.01, * * * * p<0.0001, 2-way ANOVA, Tukey

Article Snippet: Bound peptide was detected with mouse anti-V5 antibody (R960-25, ThermoFisher; 1:700 in casein buffer, 45 min, room temperature), followed by Leica post-primary rabbit anti-mouse antibody (8 min) and goat anti-rabbit HRP conjugate (8 min), with PBS washes between each step.

Techniques: Staining

Journal: bioRxiv

Article Title: Glycan Profiling Identifies Chondroitin-4-sulfate as a Biomarker for Platinum Response and Therapeutic Target in Ovarian Cancer

doi: 10.1101/2025.10.06.675352

Figure Lengend Snippet: (A) UVA CHTN OC TMA; Representative samples of patient OC subtypes (clinical history unknown) and (B) normal tissues stained with rVAR2-V5 protein. (C) UVA CHTN OC TMA; Percentage of sample area staining positively for rVAR2-V5. Values are representative of the mean of 4 cores per patient sample. (D) UVA CHTN OC TMA; Percentage of TMA samples above the cut-off score. (E) UPenn CCC TMA samples; Percentage of sample area staining positively for rVAR2-V5.

Article Snippet: Bound peptide was detected with mouse anti-V5 antibody (R960-25, ThermoFisher; 1:700 in casein buffer, 45 min, room temperature), followed by Leica post-primary rabbit anti-mouse antibody (8 min) and goat anti-rabbit HRP conjugate (8 min), with PBS washes between each step.

Techniques: Staining

Journal: medRxiv

Article Title: Human Kidney is a Target for Novel Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Infection

doi: 10.1101/2020.03.04.20031120

Figure Lengend Snippet: The expression of viral NP antigen was detected in kidney ( A ∼ C ) and lung ( D ) tissues from COVID-19 patients undergoing postmortem examination. A : arrow indicated NP positive tubules and arrow head indicated viral inclusion body. B : arrow indicated viral NP positive cells dropped from normal tubule; C : arrow indicated NP positive cells and circle indicated glomerulus; E: Absence expression of viral NP protein in normal kidney tissues; F: Sections incubated with rabbit IgG1 isotype control antibodies. Scale bar= 100 μM.

Article Snippet: The sections were then incubated overnight at 4 °C with primary anti-SARS-CoV-2 nucleocaspid protein (NP) antibodies (clone ID: 019, 1:100, rabbit IgG; Sino Biological, Beijing), anti-CD8 (Clone ID:4B11, 1:100, mouse IgG2b; BIO-RAD), anti-CD68 (Clone ID:KP1, 1:100, mouse IgG1; BIO-RAD), anti-CD56 (Clone ID:123C3, 1:100, mouse IgG1; BIO-RAD), anti-C5b-9 (clone ID: aE11, 1:100, mouse IgG; Dakocytomation) or rabbit-isotype antibody controls (1:100; Dako).

Techniques: Expressing, Incubation

Journal: medRxiv

Article Title: Human Kidney is a Target for Novel Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Infection

doi: 10.1101/2020.03.04.20031120

Figure Lengend Snippet: The expression of (A) CD68, CD8 and CD56; (B) C5b-9 in kidney tissues from COVID-19 patients undergoing postmortem examination or normal healthy was detected by immunohistochemistry. Arrow indicated positive cells. Scale bar= 100 μM.

Article Snippet: The sections were then incubated overnight at 4 °C with primary anti-SARS-CoV-2 nucleocaspid protein (NP) antibodies (clone ID: 019, 1:100, rabbit IgG; Sino Biological, Beijing), anti-CD8 (Clone ID:4B11, 1:100, mouse IgG2b; BIO-RAD), anti-CD68 (Clone ID:KP1, 1:100, mouse IgG1; BIO-RAD), anti-CD56 (Clone ID:123C3, 1:100, mouse IgG1; BIO-RAD), anti-C5b-9 (clone ID: aE11, 1:100, mouse IgG; Dakocytomation) or rabbit-isotype antibody controls (1:100; Dako).

Techniques: Expressing, Immunohistochemistry